RESUMO
In this paper, measurements of the optical properties (diffuse reflectance, total and collimated transmittance) of brain tissues in healthy rats and rats with C6-glioma were performed in the spectral range from 350 to 1800 nm. Using these measurements, characteristic tissue optical parameters, such as absorption coefficient, scattering coefficient, reduced scattering coefficient, and scattering anisotropy factor were reconstructed. It was obtained that the 10-day development of glioma led to increase of absorption coefficient, which was associated with the water content elevation in the tumor. However, further development of the tumor (formation of the necrotic core) led to decrease in the water content. The dependence of the scattering properties on the different stages of model glioma development was more complex. Light penetration depth into the healthy and tumor brain was evaluated.
RESUMO
The laser speckle contrast analysis (LASCA) is one of the most applicable tools in microcirculation studies. While the basic idea, as well as experimental setup for this method, are fairly simple, there is still the room for advancing of data processing algorithms. Specifically, the conventional realizations of LASCA method may limit the spatial and/or temporal resolution and thus fail in the detection of very small contrast objects since they based on the fixed-size rectangular sliding window function. We suggest an alternative data processing algorithm based on the usage of the Gaussian sliding filter for a sequential determination of both spatial and temporal parts of the speckle contrast. The suggested replacement of conventional box filter leads to the monotonic damping of high-frequency spectral components that results in a better elimination of ringing and aliasing effects in the spatio-temporal speckle contrast outputs. Additionally, we show that such sliding filtration increases robustness with respect to the processing of a sequence of nonstabilised images. We support this consideration with representative examples of processing both surrogate and real experimental data.
Assuntos
Algoritmos , Processamento de Imagem Assistida por Computador , Vasos Sanguíneos/anatomia & histologia , Vasos Sanguíneos/fisiologia , Humanos , Microcirculação/fisiologiaRESUMO
Nowadays, dynamically developing optical (photonic) technologies play an ever-increasing role in medicine. Their adequate and effective implementation in diagnostics, surgery, and therapy needs reliable data on optical properties of human tissues, including skin. This paper presents an overview of recent results on the measurements and control of tissue optical properties. The issues reported comprise a brief review of optical properties of biological tissues and efficacy of optical clearing (OC) method in application to monitoring of diabetic complications and visualization of blood vessels and microcirculation using a number of optical imaging technologies, including spectroscopic, optical coherence tomography, and polarization- and speckle-based ones. Molecular modeling of immersion OC of skin and specific technique of OC of adipose tissue by its heating and photodynamic treatment are also discussed.